Brett Lewis, QUT, QLD “The Corals Beneath” This video is of a lone coral polyp in a sea of thousands of clones that normally make up a colonial reef-building coral. It shows the coral polyp, its cells and the structures that would generally be hidden from standard imaging techniques - including the polyps retractor muscles, gastrodermal cavity, mesenteries and mesenterial filaments – and all in three dimensions. The image was taken on a Nikon A1r inverted confocal fluorescence microscope with excitation lasers in the DAPI, FITC, TRITC, and Cy5 range. This is a Z-stacked image made up of approx. 60 images taken over 36 hours. Images are of the corals natural autofluorescence emitting in Cyan (CFP's), Green (GFP's), Red and near infra-red (RFP's). Orange/pink is the coral's symbiotic algae, blue and green and the corals supporting cells and mucocytes, and the red cells in the tentacle tips are the stinging nematocysts.